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Image Search Results
Journal: Traffic (Copenhagen, Denmark)
Article Title: Components of the G s signaling cascade exhibit distinct changes in mobility and membrane domain localization upon β 2 -adrenergic receptor activation
doi: 10.1111/tra.12724
Figure Lengend Snippet: SMM tracking and colocalization image analysis. The original stack of images of single molecules (in this example Halo-Gγ2) undergoes the process of molecule localization detection and tracking using the Trackmate Fiji plugin. Diffusion coefficient values are calculated using @msdanalyzer MATLAB-based software. The original ensemble TIRF image of clathrin light chain-mNeonGreen (CLC-mNeonGreen) or caveolin-1-mNeonGreen (Cav1-mNeonGreen) undergoes background subtraction and channel drift correction. The image is then thresholded using Niblack’s local thresholding algorithm and cell mask creation in Fiji. All localizations outside the mask are excluded from analysis. SMM tracking results are overlaid with detected clathrin-coated structures (CCSs) or caveolae and colocalization coefficients and membrane domain residence times are determined using in-house built MATLAB scripts. Scale bar 10 μm.
Article Snippet: 41 Diffusion coefficients for individual tracks were calculated by analyzing the results of the
Techniques: Diffusion-based Assay, Software, Membrane